A receptor tyrosine kinase found in breast carcinoma cells has an extracellular discoidin I-like domain

Proc Natl Acad Sci U S A. 1993 Jun 15;90(12):5677-81. doi: 10.1073/pnas.90.12.5677.

Abstract

We have identified a breast carcinoma tyrosine phosphoprotein, discoidin domain receptor (DDR), that defines an unusual class of receptor tyrosine kinases. The DDR cDNA predicts a C-terminal tyrosine kinase domain and an N-terminal domain similar to the Dictyostelium discoideum lectin discoidin I. These domains are connected by an extraordinary hydrophilic proline/glycine-rich domain, which is interrupted by a predicted transmembrane sequence. This extended proline/glycine-rich region may be required for an unusual geometry of interaction with ligand or substrates. Discoidin I domains are also found in other proteins, including coagulation factors V and VIII, and may represent a class of domains that interact with specific cell surface molecules.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Breast Neoplasms / enzymology*
  • Cell Line
  • Dictyostelium / genetics
  • Discoidins
  • Fungal Proteins / genetics*
  • Gene Expression
  • Humans
  • Lectins / genetics
  • Molecular Sequence Data
  • Oligonucleotides, Antisense
  • Protein-Tyrosine Kinases / biosynthesis
  • Protein-Tyrosine Kinases / genetics*
  • Protozoan Proteins*
  • Receptors, Cell Surface / biosynthesis
  • Receptors, Cell Surface / genetics*
  • Sequence Homology, Amino Acid
  • Transfection
  • Tumor Cells, Cultured

Substances

  • Discoidins
  • Fungal Proteins
  • Lectins
  • Oligonucleotides, Antisense
  • Protozoan Proteins
  • Receptors, Cell Surface
  • Protein-Tyrosine Kinases

Associated data

  • GENBANK/L11315