Detection of hepatitis A virus in concentrated river water by polymerase chain reaction

Zentralbl Hyg Umweltmed. 1993 Mar;193(6):521-7.

Abstract

Sensitive and specific techniques are needed to detect Hepatitis A virus in environmental samples. PCR was used on concentrated river samples. In the analysis of 13 samples, two were positive by Elisa (15.3%), three were positive on cell culture (23%), five were positive by hybridization (38.4%) and height with PCR (67%).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • DNA Probes
  • DNA, Viral / genetics
  • DNA, Viral / isolation & purification
  • Fresh Water
  • Hepatovirus / genetics
  • Hepatovirus / isolation & purification*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides
  • Polymerase Chain Reaction / methods*
  • Water Microbiology*

Substances

  • DNA Probes
  • DNA, Viral
  • Oligodeoxyribonucleotides