Conservation of T cell receptor usage by HLA B27-restricted influenza-specific cytotoxic T lymphocytes suggests a general pattern for antigen-specific major histocompatibility complex class I-restricted responses

Eur J Immunol. 1993 Jul;23(7):1417-21. doi: 10.1002/eji.1830230702.


Eight HLA B27-restricted influenza A virus nucleoprotein 383-391-specific cytotoxic T lymphocyte (CTL) clones were obtained from three unrelated donors following natural infection. T cell receptor (TcR) usage was studied using the "anchored" polymerase chain reaction. TcR alpha-chain usage was restricted with three predominant V alpha (V alpha 12.1, 14.1, 22) and two predominant J alpha segments. beta-chain variable-region usage was also conserved, with V beta 7 being used by five clones despite contributing less than 2% of peripheral blood lymphocyte V beta sequences of one individual studied. The TcR beta-chain junctional region was highly conserved even between CTL clones from unrelated individuals, with a negatively charged amino acid, contributed to by N-region addition, encoded at position 97 in all but two clones. This study shows that peptide-specific HLA B27-restricted CTL following influenza virus infection use very similar TcR and, when considered with previous studies, suggests a pattern of TcR conservation for major histocompatibility complex class I-restricted responses. No difference in TcR usage was detected between one healthy donor and two with HLA B27-associated arthritis.

MeSH terms

  • Amino Acid Sequence
  • Antigens, Viral / immunology*
  • Gene Rearrangement, alpha-Chain T-Cell Antigen Receptor
  • Gene Rearrangement, beta-Chain T-Cell Antigen Receptor / genetics
  • HLA-B27 Antigen / immunology*
  • Humans
  • In Vitro Techniques
  • Influenza A virus / immunology*
  • Molecular Sequence Data
  • Nucleocapsid Proteins
  • Nucleoproteins*
  • Receptors, Antigen, T-Cell, alpha-beta / genetics*
  • Spondylitis, Ankylosing / immunology
  • T-Lymphocytes, Cytotoxic / immunology*
  • Viral Core Proteins / immunology*


  • Antigens, Viral
  • HLA-B27 Antigen
  • Nucleocapsid Proteins
  • Nucleoproteins
  • Receptors, Antigen, T-Cell, alpha-beta
  • Viral Core Proteins