Beta-adrenergic receptors in human anterior optic nerve: an autoradiographic study

Eye (Lond). 1993;7 ( Pt 1):122-6. doi: 10.1038/eye.1993.25.

Abstract

Sections of human anterior optic nerve and nerve head were incubated in a physiological solution containing a radiolabelled beta blocker at a low concentration. The beta blocker used was (-)-(125iodo)-cyanopindolol, which has a high affinity and specificity for beta-adrenergic receptors. Concurrent incubations were performed with a great excess of unlabelled beta blocker added to demonstrate non-specific binding. Following incubation the sections were washed and dried. They were then apposed to photographic film for 5 days and developed. Incubations were performed with the stereoisomers of propranolol and an alpha blocker as well as specific beta-one and beta-two blockers. Beta-adrenergic receptors were demonstrated in anterior optic nerve and optic nerve head. The majority were of the beta-two subtype.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Autoradiography
  • Humans
  • Iodocyanopindolol
  • Optic Disk / chemistry
  • Optic Nerve / chemistry*
  • Pindolol / analogs & derivatives
  • Pindolol / metabolism
  • Propranolol / metabolism
  • Receptors, Adrenergic, beta / analysis*
  • Receptors, Adrenergic, beta / metabolism

Substances

  • Receptors, Adrenergic, beta
  • Iodocyanopindolol
  • Propranolol
  • Pindolol