Transformation with katG restores isoniazid-sensitivity in Mycobacterium tuberculosis isolates resistant to a range of drug concentrations

Mol Microbiol. 1993 May;8(3):521-4. doi: 10.1111/j.1365-2958.1993.tb01596.x.

Abstract

Isoniazid-resistant isolates of Mycobacterium tuberculosis were transformed with a plasmid vector carrying the functional catalase-peroxidase (katG) gene. Expression of katG restored full drug susceptibility in isolates initially resistant to concentrations ranging from 3.2 to > 50 micrograms ml-1. Transformation with the corresponding katG gene from Escherichia coli resulted in low-level expression of catalase and peroxidase activities and conferred partial isoniazid sensitivity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins*
  • Catalase / genetics
  • Catalase / metabolism*
  • Drug Resistance, Microbial / genetics
  • Genes, Bacterial
  • Isoniazid / pharmacology*
  • Mycobacterium tuberculosis / drug effects*
  • Mycobacterium tuberculosis / metabolism
  • Peroxidase / genetics
  • Peroxidase / metabolism*
  • Peroxidases / genetics
  • Peroxidases / metabolism*
  • Plasmids
  • Recombinant Fusion Proteins / metabolism*
  • Transformation, Bacterial

Substances

  • Bacterial Proteins
  • Recombinant Fusion Proteins
  • Peroxidases
  • Catalase
  • catalase HPI
  • Peroxidase
  • Isoniazid