Skip to main page content
Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
, 90 (13), 6228-32

Cloning, Chromosomal Localization, and Functional Expression of the Alpha 1 Subunit of the L-type Voltage-Dependent Calcium Channel From Normal Human Heart

Affiliations

Cloning, Chromosomal Localization, and Functional Expression of the Alpha 1 Subunit of the L-type Voltage-Dependent Calcium Channel From Normal Human Heart

D Schultz et al. Proc Natl Acad Sci U S A.

Abstract

A unique structural variant of the cardiac L-type voltage-dependent calcium channel alpha 1 subunit cDNA was isolated from libraries derived from normal human heart mRNA. The deduced amino acid sequence shows significant homology to other calcium channel alpha 1 subunits. However, differences from the rabbit heart alpha 1 include a shortened N-terminus, a unique C-terminal insertion, and both forms of an alternatively spliced motif IV S3 region. The shortened N-terminus provides optimal access to consensus sequences thought to facilitate translation. Northern blot analysis revealed a single hybridizing mRNA species of 9.4 kb. The gene for the human heart alpha 1 subunit was localized specifically to the distal region of chromosome 12p13. The cloned alpha 1 subunit was expressed in Xenopus oocytes and single-channel analyses revealed native-like pharmacology and channel properties.

Similar articles

See all similar articles

Cited by 27 PubMed Central articles

See all "Cited by" articles

References

    1. Genomics. 1991 Dec;11(4):914-9 - PubMed
    1. J Biol Chem. 1992 Jan 25;267(3):1792-7 - PubMed
    1. Proc Natl Acad Sci U S A. 1992 May 15;89(10):4628-32 - PubMed
    1. Genomics. 1992 Jun;13(2):355-62 - PubMed
    1. Proc Natl Acad Sci U S A. 1977 Dec;74(12):5463-7 - PubMed

Publication types

Associated data

Feedback