Abstract
Autocrine motility factor (AMF) is a 55 kDa cytokine which is produced and secreted by cancer cells and which regulates cell motility via binding to its receptor, a 78 kDa cell surface glycoprotein (gp78-AMFR), and activating a pertussis toxin (PT)-sensitive G-protein. AMF purified from HT-1080 human fibrosarcoma cells stimulates the growth and motility of 3T3-A31-fibroblasts at a concentration of 0.1 ng/ml or less. The expression of total as well as cell surface gp78-AMFR is down-regulated in contact-inhibited A31-fibroblasts and AMF stimulates the healing of experimentally wounded, density-arrested A31 monolayer cultures. This is the first report of the paracrine and mitogenic actions of AMF and the results presented here show that AMF functions as a growth factor and suggest a possible role for its activity in normal tissue regeneration and tumor cell dissemination.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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3T3 Cells
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Animals
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Cell Communication / drug effects
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Cell Division / drug effects
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Cell Movement / drug effects*
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DNA / biosynthesis
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DNA Replication / drug effects
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Dose-Response Relationship, Drug
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Fibroblasts / cytology
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Fibroblasts / drug effects
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Fibroblasts / physiology
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Fibrosarcoma
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Glucose-6-Phosphate Isomerase / isolation & purification
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Glucose-6-Phosphate Isomerase / metabolism
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Glucose-6-Phosphate Isomerase / pharmacology*
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Growth Substances / isolation & purification
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Growth Substances / metabolism
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Growth Substances / pharmacology*
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Humans
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Kinetics
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Melanoma, Experimental
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Mice
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Receptors, Autocrine Motility Factor
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Receptors, Cell Surface / metabolism
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Receptors, Cytokine*
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Time Factors
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Tumor Cells, Cultured
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Ubiquitin-Protein Ligases
Substances
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Growth Substances
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Receptors, Cell Surface
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Receptors, Cytokine
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DNA
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AMFR protein, human
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Amfr protein, mouse
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Receptors, Autocrine Motility Factor
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Ubiquitin-Protein Ligases
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Glucose-6-Phosphate Isomerase