Bovine chromaffin cells possess FTX-sensitive calcium channels

Biochem Biophys Res Commun. 1993 Jul 30;194(2):671-6. doi: 10.1006/bbrc.1993.1874.

Abstract

The effects of the synthetic analogue of the toxin from the venom of the funnel-web spider Agenelopsis aperta (sFTX) on whole-cell Ba2+ currents through Ca2+ channels were studied in cultured bovine chromaffin cells. sFTX selectively and reversibly blocked a significant component (55 +/- 3%) of the whole-cell IBa. Effects of sFTX were additive to those of omega-conotoxin GVIA, a selective blocker of N-type Ca2+ channels, and those of furnidipine, a novel dihydropyridine L-type Ca2+ channel blocker. We conclude that the cultured bovine chromaffin cells, in addition to N- and L-type Ca2+ channels, possess a P-type component in their whole-cell currents through their Ca2+ channels.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adrenal Medulla / drug effects
  • Adrenal Medulla / physiology*
  • Animals
  • Barium / pharmacology
  • Calcium Channel Blockers / pharmacology*
  • Calcium Channels / drug effects
  • Calcium Channels / physiology*
  • Cattle
  • Cells, Cultured
  • Dihydropyridines / pharmacology
  • Dose-Response Relationship, Drug
  • Ion Channel Gating / drug effects
  • Kinetics
  • Peptides / pharmacology
  • Polyamines / pharmacology*
  • omega-Conotoxins*

Substances

  • Calcium Channel Blockers
  • Calcium Channels
  • Dihydropyridines
  • FTX, spider toxin
  • Peptides
  • Polyamines
  • omega-Conotoxins
  • Conus magus toxin
  • Barium
  • furnidipine