The cloning and expression of a sodium channel beta 1-subunit cDNA from human brain

Hum Mol Genet. 1993 Jun;2(6):745-9. doi: 10.1093/hmg/2.6.745.


Electrical excitability of neurons and muscle cells is mediated largely through the actions of the voltage-gated sodium channel. Initiation and propagation of the action potential is a direct result of the precisely controlled inward flux of sodium through these channels. Much attention has been paid to the sodium channel alpha-subunit, the major, pore-forming component. However, alpha-subunits are associated with one or more smaller beta-subunits, which have been implicated in the critical fine tuning of the gating properties of the channel. To investigate the properties of the beta-subunit, we have isolated a cDNA encoding the human brain beta 1-subunit and assigned the corresponding gene to chromosome 19. We have also examined the effects of expressing the brain beta 1-subunit on the kinetics of a coexpressed muscle sodium channel alpha-subunit. Our results underscore the functional importance of the beta 1-subunit and imply a conserved mechanism for the interaction of the beta 1-subunit with different alpha-subunits.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain Chemistry*
  • Cloning, Molecular
  • DNA / genetics*
  • Gene Expression Regulation
  • Humans
  • Hybrid Cells
  • Kinetics
  • Molecular Sequence Data
  • Muscle Proteins / biosynthesis
  • Muscle Proteins / genetics
  • Nerve Tissue Proteins / biosynthesis
  • Nerve Tissue Proteins / genetics*
  • Oocytes
  • Rats
  • Recombinant Proteins / biosynthesis
  • Recombinant Proteins / genetics
  • Sodium Channels / genetics*
  • Sodium Channels / metabolism
  • Xenopus


  • Muscle Proteins
  • Nerve Tissue Proteins
  • Recombinant Proteins
  • Sodium Channels
  • DNA

Associated data

  • GENBANK/L10338