Member of the CREB/ATF protein family, but not CREB alpha plays an active role in BLV tax trans activation in vivo

Nucleic Acids Res. 1993 Aug 11;21(16):3677-82. doi: 10.1093/nar/21.16.3677.

Abstract

The trans activator protein of Bovine Leukaemia Virus (tax) increases the rate of transcription from the virus promoter through 21 bp sequences located in three tandem copies in the virus LTR. Based on data obtained by three different experimental approaches we concluded that the central CRE-like motif found in each of the BLV 21 bp repeats plays an important and indispensable role in tax mediated trans activation. These include (i) in vivo analysis of the function of mutant 21 bp sequences in transient transfection, (ii) gel mobility shift assay to show that CREB binds to BLV 21 bp repeats in vitro and (iii) the demonstration that the production of antisense CREB mRNA inhibits tax trans activation. Further studies with different deletion mutant CREB proteins suggest that although CREB alpha can interact with factors involved in BLV trans activation, it does not promote transcription initiation; consequently some other member/s of the CREB/ATF family must be involved.

MeSH terms

  • Activating Transcription Factors
  • Base Sequence
  • Blood Proteins / metabolism*
  • Cyclic AMP Response Element-Binding Protein / biosynthesis
  • Cyclic AMP Response Element-Binding Protein / metabolism*
  • DNA, Viral / metabolism
  • Gene Products, tax / metabolism*
  • HeLa Cells
  • Humans
  • Leukemia Virus, Bovine / genetics
  • Leukemia Virus, Bovine / metabolism*
  • Molecular Sequence Data
  • Mutagenesis
  • Protein Binding
  • Repetitive Sequences, Nucleic Acid
  • Restriction Mapping
  • Transcription Factors / metabolism*
  • Transcriptional Activation*

Substances

  • Activating Transcription Factors
  • Blood Proteins
  • Cyclic AMP Response Element-Binding Protein
  • DNA, Viral
  • Gene Products, tax
  • Transcription Factors