Molecular cloning reveals the existence of a fourth gamma subunit of the vertebrate brain GABAA receptor

FEBS Lett. 1993 Oct 4;331(3):211-6. doi: 10.1016/0014-5793(93)80339-v.


We have isolated a cDNA, from the chicken, that encodes a fourth type of gamma subunit of the vertebrate brain GABAA receptor. The mature polypeptide (which we name gamma 4) displays 67%, 69% and 70% identity, respectively, to the rat gamma 1, gamma 2 and gamma 3 subunits. In the developing chicken brain, the gamma 4-subunit mRNA is first detected at embryonic day 13; the transcript level then increases progressively during embryogenesis. In situ hybridization reveals that the gamma 4-subunit mRNA is abundant in several brain regions, including the ectostriatum, nucleus rotundus and hyperstriatum ventrale, which are involved in visual processing and learning.

Publication types

  • Comparative Study

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brain / embryology
  • Chick Embryo
  • Cloning, Molecular
  • Gene Expression
  • Genes
  • In Situ Hybridization
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • RNA, Messenger / genetics
  • Rats
  • Receptors, GABA-A / genetics*
  • Sequence Alignment


  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • Receptors, GABA-A

Associated data

  • GENBANK/X73533