Sprint training increases human skeletal muscle Na(+)-K(+)-ATPase concentration and improves K+ regulation

J Appl Physiol (1985). 1993 Jul;75(1):173-80. doi: 10.1152/jappl.1993.75.1.173.


This study investigated the effects of sprint training on muscle Na(+)-K(+)-adenosinetriphosphatase (ATPase) concentration, plasma [K+] regulation, muscle performance, and fatigue during severe intermittent exercise. Six untrained male subjects underwent intensive cycle-sprint training for 7 wk. Muscle biopsies were taken at rest from the vastus lateralis muscle before and after 7 wk of training and were assayed for Na(+)-K(+)-ATPase concentration using vanadate-facilitated [3H]ouabain binding to intact samples. Before and after the training period, subjects performed four maximal 30-s exercise bouts (EB) on a cycle ergometer, each separated by a 4-min recovery. Arterialized venous blood samples were drawn immediately before and after each sprint bout and were analyzed for plasma [K+]. The work output was significantly elevated (11%) across all four EBs after training. The muscle [3H]ouabain binding site concentration was significantly increased (16%) from 333 +/- 19 to 387 +/- 15 (SE) pmol/g wet wt after training but was unchanged in muscle obtained from three control subjects. Plasma [K+] rose by 1-2 mmol/l with each EB and declined rapidly by the end of each recovery period. The increases in plasma [K+] resulting from each EB were significantly lower (19%) after training. The ratios of rise in plasma [K+] relative to work output during each EB were also significantly lower (27%) after training. The increased muscle [3H]ouabain binding site concentration and the reduced ratio of rise in [K+] relative to work output with exercise are both consistent with improved plasma and skeletal muscle K+ regulation after sprint training.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Blood Gas Analysis
  • Body Weight / physiology
  • Fatigue / physiopathology
  • Hematocrit
  • Hemoglobins / metabolism
  • Humans
  • Male
  • Muscles / enzymology*
  • Muscles / physiology
  • Ouabain / metabolism
  • Physical Education and Training*
  • Physical Exertion / physiology
  • Potassium / blood
  • Potassium / physiology*
  • Receptors, Drug / metabolism
  • Sodium-Potassium-Exchanging ATPase / metabolism*


  • Hemoglobins
  • Receptors, Drug
  • cardiac glycoside receptors
  • Ouabain
  • Sodium-Potassium-Exchanging ATPase
  • Potassium