Stimulation of nerve growth factor synthesis/secretion in mouse astroglial cells by coenzymes

Biochem Mol Biol Int. 1993 Jul;30(4):615-21.

Abstract

We examined the effect of coenzymes such as PQQ, pyrroloquinoline quinone; TOPA, 3-(2,4,5-trihydroxyphenyl)-DL-alanine, and lipoic acid on nerve growth factor (NGF) synthesis in mouse astroglial cells, BALB c/3T3 cells, and WS-1 cells in culture. These coenzymes had a stimulating effect on NGF synthesis without causing cytotoxicity. Especially PQQ showed the strongest activity of promoting NGF synthesis in astroglial cells, whereas lipoic acid had the strongest effect on BALB c/3T3 cells. The activity may not due to the catechol ring or 1,4-benzoquinone ring, but due to the oxidative or reductive activity. These results suggest that these coenzymes may play a role in NGF synthesis and neuronal survival through the stimulating effect of the NGF synthesis in brain and such compounds are good candidates as NGF inducers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • 3T3 Cells
  • Animals
  • Astrocytes / cytology
  • Astrocytes / drug effects*
  • Astrocytes / metabolism
  • Cell Survival / drug effects
  • Cells, Cultured
  • Coenzymes / pharmacology*
  • Dihydroxyphenylalanine / analogs & derivatives*
  • Dihydroxyphenylalanine / pharmacology
  • Mice
  • Mice, Inbred BALB C
  • Nerve Growth Factors / biosynthesis*
  • Nerve Growth Factors / metabolism
  • Oxidation-Reduction
  • PQQ Cofactor
  • Quinolones / pharmacology*
  • Thioctic Acid / pharmacology*

Substances

  • Coenzymes
  • Nerve Growth Factors
  • Quinolones
  • 6-hydroxydopa
  • Dihydroxyphenylalanine
  • PQQ Cofactor
  • Thioctic Acid