A NotI restriction map of the entire long arm of human chromosome 21

Nat Genet. 1993 Aug;4(4):361-6. doi: 10.1038/ng0893-361.


A variety of maps of the human genome have been constructed, including cloned DNA maps. We have isolated 40 of the 42 NotI sites that exist on the long arm of human chromosome 21, as NotI linking clones and constructed a complete NotI restriction map spanning the entire region. This map, which provides the most reliable ordering and distance estimation in the region from a pericentromeric locus to the terminus, demonstrates the usefulness of linking clone mapping for analysing human chromosomes.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Chromosomes, Human, Pair 21*
  • Cloning, Molecular
  • DNA / analysis
  • DNA / genetics
  • Deoxyribonucleases, Type II Site-Specific / metabolism*
  • Genetic Linkage
  • Genetic Markers
  • Genome, Human
  • Humans
  • Hybrid Cells
  • Mice
  • Restriction Mapping*


  • Genetic Markers
  • DNA
  • Deoxyribonucleases, Type II Site-Specific
  • GCGGCCGC-specific type II deoxyribonucleases