Chromosomal distribution of 320 genes from a brain cDNA library

M H Polymeropoulos; H Xiao; J M Sikela; M Adams; J C Venter; C R Merril

doi:10.1038/ng0893-381

Chromosomal distribution of 320 genes from a brain cDNA library

Nat Genet. 1993 Aug;4(4):381-6. doi: 10.1038/ng0893-381.

Authors

M H Polymeropoulos¹, H Xiao, J M Sikela, M Adams, J C Venter, C R Merril

Affiliation

¹ Laboratory of Biochemical Genetics, National Institute of Mental Health, Neuroscience Center at St. Elizabeths, Washington, DC 20032.

PMID: 8401586
DOI: 10.1038/ng0893-381

Abstract

We have determined the chromosomal assignment of 320 brain expressed genes by studying the segregation of polymerase chain reaction (PCR) products in human rodent somatic cell hybrids and by genetically mapping polymorphic cDNAs using the CEPH (Centre d'Etude du Polymophisme Humaine) reference pedigrees and database. These mapped genes can function as markers on the physical map of the human genome, as well as serve as candidate disease gene loci. Distribution of these genes to the human chromosomes correlates well with the GC content of the chromosomes. However, the distribution of these genes does not correlate well with the cytogenetic length of each chromosome.

Publication types

Research Support, U.S. Gov't, Non-P.H.S.
Research Support, U.S. Gov't, P.H.S.

MeSH terms

Animals
Brain / metabolism*
Chromosome Mapping*
Chromosomes, Human*
Cloning, Molecular
DNA, Complementary / chemistry
DNA, Complementary / genetics*
Humans
Hybrid Cells
Information Systems
Lod Score
Polymerase Chain Reaction / methods
Polymorphism, Genetic

Substances

DNA, Complementary