Brain myosin-V is a two-headed unconventional myosin with motor activity

Cell. 1993 Oct 8;75(1):13-23.


Chicken myosin-V is a member of a recently recognized class of myosins distinct from both the myosins-I and the myosins-II. We report here the purification, electron microscopic visualization, and motor properties of a protein of this class. Myosin-V molecules consist of two heads attached to an approximately 30 nm stalk that ends in a globular region of unknown function. Myosin-V binds to and decorates F-actin, has actin-activated magnesium-ATPase activity, and is a barbed-end-directed motor capable of moving actin filaments at rates of up to 400 nm/s. Myosin-V does not form filaments. Each myosin-V heavy chain is associated with approximately four calmodulin light chains as well as two less abundant proteins of 23 and 17 kd.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / metabolism
  • Animals
  • Brain / metabolism*
  • Calmodulin / metabolism
  • Calmodulin-Binding Proteins / isolation & purification
  • Calmodulin-Binding Proteins / metabolism*
  • Calmodulin-Binding Proteins / ultrastructure
  • Chickens
  • Egtazic Acid / pharmacology
  • Electrophoresis, Polyacrylamide Gel
  • Freeze Etching
  • Macromolecular Substances
  • Microscopy, Electron
  • Models, Structural
  • Myosin Type V*
  • Nerve Tissue Proteins / isolation & purification
  • Nerve Tissue Proteins / metabolism*
  • Nerve Tissue Proteins / ultrastructure


  • Actins
  • Calmodulin
  • Calmodulin-Binding Proteins
  • Macromolecular Substances
  • Nerve Tissue Proteins
  • chicken brain myosin-V p190
  • Egtazic Acid
  • Myosin Type V