Variants of vasoactive intestinal peptide in mouse mast cells and rat basophilic leukemia cells

Cell Immunol. 1993 Oct 15;151(2):369-78. doi: 10.1006/cimm.1993.1246.


Radioimmunoassays for neuroendocrine vasoactive intestinal peptide (VIP1-28) detected 30-120 fmol of structurally related peptides in extracts of 10(7) mouse peritoneal mast cells, bone marrow-derived mast cells, cultured PT-18 and C1.MC/C57.1 lines of mast cells, and rat basophilic leukemia (RBL) cells. No VIP was found in peritoneal cells of mast cell-deficient WBB6F1-W/Wv mice, whereas the amounts extracted from peritoneal cells of the congenic normal (WBB6F1-+/+) mice were similar to those from cultured mouse mast cells. Sephadex G-25 gel filtration resolved two different-sized variants of VIP from mouse mast cells and RBL cells. Amino acid sequence analyses showed that the smaller variant is VIP10-28. The principal amino-terminally larger variant of VIP from C1.MC/C57.1 mouse mast cells and RBL cells exhibited amino acid sequence homology with VIP(-6)-28, and this sequence was established for the corresponding larger VIP from PT-18 mast cells. Polymerase chain reaction amplification of two different substituent sequences of prepro VIP in RBL cell RNA identified the VIP message. VIP10-28 was released from mouse mast cells concurrently with histamine by IgE-dependent stimulation. Rodent mast cell-derived VIP thus consists of both the truncated VIP10-28 and amino-terminally larger forms that appear to be generated by peptidolysis of a preproVIP similar to that found in neural cells.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cells, Cultured
  • Leukemia, Basophilic, Acute / metabolism*
  • Mast Cells / metabolism*
  • Mice
  • Mice, Inbred Strains
  • Molecular Sequence Data
  • Peptide Fragments / metabolism
  • Protein Precursors / metabolism
  • Radioimmunoassay
  • Rats
  • Tumor Cells, Cultured
  • Vasoactive Intestinal Peptide / analogs & derivatives
  • Vasoactive Intestinal Peptide / metabolism*


  • Peptide Fragments
  • Protein Precursors
  • Vasoactive Intestinal Peptide
  • vasoactive intestinal peptide (10-28)
  • preprovasoactive intestinal peptide