Mescaline-induced changes of brain-cortex ribosomes. Mescaline demethylase activity of brain-cortex soluble supernatant

Naunyn Schmiedebergs Arch Pharmacol. 1977 Feb;296(3):297-300. doi: 10.1007/BF00498697.


Brain-cortex slices demethylate mescaline and p-methoxyacetanilide, a reference O-demethylating substrate, though the rate of demethylation of mescaline is about one third that of the reference substrate. The demethylase activity is localized mostly in the soluble supernatant (105 000 x g). It is purified 47-fold with respect to the demethylation of mescaline by ammonium sulfate precipitation and DEAE cellulose chromatography. The partially purified demethylase, which is stable for 3-5 days at -5 degrees C in the presence of dithiothreitol and glutathione and is inhibited by p-chloromercuribenzoate, has maximal activity at pH between 7.2 and 8.0. It demethylates mescaline into 3,4-dimethoxy-5-hydroxyphenethylamine and 3,5-dimethoxy-4-hydroxyphenethylamine and some unidentified derivatives.

MeSH terms

  • Acetamides / analogs & derivatives
  • Acetamides / metabolism
  • Animals
  • Cerebral Cortex / enzymology
  • Cerebral Cortex / ultrastructure*
  • Goats
  • In Vitro Techniques
  • Kinetics
  • Mescaline / metabolism*
  • Oxidoreductases / metabolism*
  • Oxidoreductases, O-Demethylating / isolation & purification
  • Oxidoreductases, O-Demethylating / metabolism*
  • Subcellular Fractions / enzymology*


  • Acetamides
  • Oxidoreductases
  • Oxidoreductases, O-Demethylating
  • Mescaline