Monocyte chemoattractant protein-1 (MCP-1) in inflammatory joint diseases and its involvement in the cytokine network of rheumatoid synovium

Clin Immunol Immunopathol. 1993 Oct;69(1):83-91. doi: 10.1006/clin.1993.1153.


Macrophages infiltrated into synovium play an important role in joint destruction in inflammatory joint diseases. In this study we focused on the production of monocyte chemoattractant protein-1 (MCP-1), a recently identified monocyte chemotactic protein, by inflammatory synovium. Synovial fluid (SF) from rheumatoid arthritis (RA), osteoarthritis, gout, and traumatic arthritis contained MCP-1. MCP-1 was produced in the synovium of patients with RA and other inflammatory joint disease in in vitro culture systems; differences in the amounts produced were not significant. Synovial MCP-1 production in RA was further investigated. Levels of MCP-1 were significantly correlated with levels of IL-1 beta, IL-6, and IL-8 in the culture supernatants of synovia from RA. Using immunohistochemical techniques, MCP-1 was detected in the lining and sublining cells and in the vascular endothelial cells of rheumatoid synovia. Rheumatoid synovia with active inflammation were stained more intensely by anti-MCP-1 antibody than were those with weak or inactive inflammation. IL-1 beta and TNF-alpha stimulated the expression of MCP-1 mRNA and de novo MCP-1 synthesis by cultured synovial cells. These results suggest the production of MCP-1 by synovium of various inflammatory joint diseases. In rheumatoid synovium, a cytokine network involving MCP-1 and other proinflammatory cytokines (IL-1 beta, IL-6, IL-8, and TNF-alpha) contributes to the immunopathogenesis of RA.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arthritis / immunology
  • Arthritis / metabolism*
  • Arthritis, Rheumatoid / immunology
  • Arthritis, Rheumatoid / metabolism
  • Cells, Cultured
  • Chemokine CCL2
  • Chemotactic Factors / analysis
  • Chemotactic Factors / biosynthesis*
  • Cytokines / biosynthesis*
  • Cytokines / immunology
  • Humans
  • Immunohistochemistry
  • Interleukin-1 / pharmacology
  • Organ Culture Techniques
  • RNA, Messenger / genetics
  • Stimulation, Chemical
  • Synovial Fluid / chemistry
  • Synovial Membrane / cytology
  • Synovial Membrane / immunology
  • Synovial Membrane / metabolism*
  • Synovitis / immunology
  • Synovitis / metabolism
  • Tumor Necrosis Factor-alpha / pharmacology


  • Chemokine CCL2
  • Chemotactic Factors
  • Cytokines
  • Interleukin-1
  • RNA, Messenger
  • Tumor Necrosis Factor-alpha