A Saccharomyces cerevisiae homologue of mammalian translation initiation factor 4B contributes to RNA helicase activity

EMBO J. 1993 Oct;12(10):3997-4003.

Abstract

The TIF3 gene of Saccharomyces cerevisiae was cloned and sequenced. The deduced amino acid sequence shows 26% identity with the sequence of mammalian translation initiation factor eIF-4B. The TIF3 gene is not essential for growth; however, its disruption results in a slow growth and cold-sensitive phenotype. In vitro translation of total yeast RNA in an extract from a TIF3 gene-disrupted strain is reduced compared with a wild-type extract. The translational defect is more pronounced at lower temperatures and can be corrected by the addition of wild-type extract or mammalian eIF-4B, but not by addition of mutant extract. In vivo translation of beta-galactosidase reporter mRNA with varying degree of RNA secondary structure in the 5' leader region in a TIF3 gene-disrupted strain shows preferential inhibition of translation of mRNA with more stable secondary structure. This indicates that Tif3 protein is an RNA helicase or contributes to RNA helicase activity in vivo.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Blotting, Northern
  • Blotting, Southern
  • DNA, Fungal
  • Eukaryotic Initiation Factor-3
  • Eukaryotic Initiation Factors*
  • Fungal Proteins / genetics*
  • Fungal Proteins / metabolism
  • Genes, Fungal
  • Kinetics
  • Molecular Sequence Data
  • Peptide Initiation Factors / genetics*
  • Peptide Initiation Factors / metabolism
  • Protein Biosynthesis*
  • RNA Helicases
  • RNA Nucleotidyltransferases / genetics*
  • RNA Nucleotidyltransferases / metabolism*
  • Saccharomyces cerevisiae / enzymology
  • Saccharomyces cerevisiae / genetics*
  • Saccharomyces cerevisiae Proteins*
  • Sequence Homology, Amino Acid

Substances

  • DNA, Fungal
  • Eukaryotic Initiation Factor-3
  • Eukaryotic Initiation Factors
  • Fungal Proteins
  • Peptide Initiation Factors
  • Saccharomyces cerevisiae Proteins
  • TIF3 protein, S cerevisiae
  • eIF-4B
  • RNA Nucleotidyltransferases
  • RNA Helicases