Cloning of two related genes encoding the 56-kDa and 123-kDa subunits of trehalose synthase from the yeast Saccharomyces cerevisiae

Eur J Biochem. 1993 Sep 15;216(3):849-61. doi: 10.1111/j.1432-1033.1993.tb18207.x.


Preparations of intact trehalose synthase contain three polypeptides with molecular masses of 56, 102 and 123 kDa. We have cloned the genes TSS1 and TSL1 coding for the 56- and 123-kDa subunits, respectively. These genes are located on chromosomes II (TSS1) and XIII (TSL1). The TSS1 gene was found to be identical with CIF1, a gene required for normal growth on glucose. The product of the entire TSS1 gene exhibits 37% identity with a 502-amino-acid stretch from the middle of the TSL1 product. Disruption of the TSS1 gene in yeast eliminates both trehalose 6-phosphate synthase (Tre6P synthase) and trehalose 6-phosphate phosphatase (Tre6Pase) activities, and reintroduction of this gene restores these activities. Transformation of Escherichia coli with TSS1 increases its Tre6P synthase activity. Specific proteolytic degradation of the 123-kDa polypeptide from the N-terminus greatly influences the Tre6P synthase activity, decreasing its inhibition by phosphate and activatability by fructose 6-phosphate but has little effect on the Tre6Pase activity. These results suggest that this N-terminal part confers regulatory properties upon the Tre6P synthase activity.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Cloning, Molecular
  • DNA, Fungal
  • Enzyme Activation
  • Escherichia coli
  • Genes, Fungal
  • Genetic Complementation Test
  • Glucose / metabolism
  • Glucosyltransferases / antagonists & inhibitors
  • Glucosyltransferases / genetics*
  • Glucosyltransferases / metabolism
  • Molecular Sequence Data
  • Mutation
  • Phosphoric Monoester Hydrolases / genetics
  • Phosphoric Monoester Hydrolases / metabolism
  • Plasmids
  • Saccharomyces cerevisiae / enzymology*
  • Sequence Homology, Amino Acid
  • Trypsin


  • DNA, Fungal
  • Glucosyltransferases
  • trehalose-6-phosphate synthase
  • trehalose-phosphatase
  • Phosphoric Monoester Hydrolases
  • Trypsin
  • Glucose

Associated data

  • GENBANK/M88172
  • GENBANK/X72788
  • GENBANK/Z25874
  • GENBANK/Z25876
  • GENBANK/Z25877
  • GENBANK/Z25878
  • GENBANK/Z25879
  • GENBANK/Z25880
  • GENBANK/Z25881
  • GENBANK/Z25882
  • GENBANK/Z25883