Two-dimensional 1H NMR spectra of ferricytochrome c551 from Pseudomonas aeruginosa

J M Moratal; A Donaire; J Salgado; H R Jiménez; J Castells; M Piccioli

doi:10.1016/0014-5793(93)80140-p

Two-dimensional 1H NMR spectra of ferricytochrome c551 from Pseudomonas aeruginosa

FEBS Lett. 1993 Jun 21;324(3):305-8. doi: 10.1016/0014-5793(93)80140-p.

Authors

J M Moratal¹, A Donaire, J Salgado, H R Jiménez, J Castells, M Piccioli

Affiliation

¹ Department of Inorganic Chemistry, University of Valencia, Italy.

PMID: 8405371
DOI: 10.1016/0014-5793(93)80140-p

Abstract

The full assignment of 1H NMR signals of heme proton resonances of ferricytochrome c551 from Pseudomonas aeruginosa has been performed by means of 2D NMR experiments. This technique allows the complete and unequivocal assignment of all heme resonances, including methylene resonances of the propionic groups, directly implicated in the pH dependence of the redox properties of cytochrome c551.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Bacterial Proteins / chemistry
Cytochrome c Group / chemistry*
Ferric Compounds
Heme / chemistry
Hydrogen-Ion Concentration
Magnetic Resonance Spectroscopy
Pseudomonas aeruginosa / chemistry*

Substances

Bacterial Proteins
Cytochrome c Group
Ferric Compounds
Heme
cytochrome C(551)