Differential expression of transcripts from syb, a Drosophila melanogaster gene encoding VAMP (synaptobrevin) that is abundant in non-neuronal cells

Gene. 1993 Sep 15;131(2):175-81. doi: 10.1016/0378-1119(93)90291-a.

Abstract

VAMP (synaptobrevin) is a highly conserved membrane protein originally described as a component of brain synaptic vesicles. The Drosophila melanogaster VAMP-encoding gene (syb) comprises five exons. Splicing exons 1,2,3,4,5 (syb-b) results in a protein with a C-terminal hydrophobic domain and a negligible intraluminal domain. Splicing exons 1,2,3,5 (syb-a) predicts a protein with a 20-amino-acid luminal domain at the C terminus. The ratio of syb-a to syb-b transcripts is highly regulated during development. The syb transcripts show no enrichment in the nervous system and are present in very early embryos, well before neurogenesis. The greatest concentration of syb transcripts was found in cells of the gut and malpighian tubules. Thus, syb may have a general role in membrane trafficking and, perhaps, a role in the secretion of digestive enzymes.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Alternative Splicing
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Membrane / metabolism
  • DNA
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Larva
  • Membrane Proteins / genetics*
  • Membrane Proteins / physiology
  • Molecular Sequence Data
  • Nerve Tissue Proteins / genetics*
  • Nerve Tissue Proteins / physiology
  • R-SNARE Proteins
  • RNA, Messenger / metabolism
  • Rats
  • Sequence Homology, Amino Acid

Substances

  • Membrane Proteins
  • Nerve Tissue Proteins
  • R-SNARE Proteins
  • RNA, Messenger
  • DNA

Associated data

  • GENBANK/L03653
  • GENBANK/L03654
  • GENBANK/L07632
  • GENBANK/L07643
  • GENBANK/L14270
  • GENBANK/M96932
  • GENBANK/M96933
  • GENBANK/S65693
  • GENBANK/S65694
  • GENBANK/X54209