D21S418E identifies a cAMP-regulated gene located on chromosome 21q22.3 that is expressed in placental syncytiotrophoblast and choriocarcinoma cells

Genomics. 1993 Jul;17(1):256-9. doi: 10.1006/geno.1993.1317.

Abstract

A partial cDNA (D21S418E) whose nucleotide sequence has no significant homologies with known mammalian DNA sequences was isolated from a human placental library. The cDNA hybridized with a 10-kb transcript present in term placenta. Messages of 10 and 7.5 kb were induced in BeWo and JEG-3 choriocarcinoma cells by treatment with 8-Br-cAMP. The mRNA was not detected in human brain, liver, lung, kidney, pancreas, heart, skeletal muscle, or myometrium. The D21S418E locus was assigned to a 3.5-Mb region of chromosome 21q22.3.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • 8-Bromo Cyclic Adenosine Monophosphate / pharmacology
  • Animals
  • Base Sequence
  • Choriocarcinoma / genetics*
  • Choriocarcinoma / metabolism
  • Choriocarcinoma / pathology
  • Chromosome Mapping
  • Chromosomes, Human, Pair 21*
  • Cricetinae
  • Cricetulus
  • Cyclic AMP / physiology*
  • DNA, Complementary / genetics
  • Female
  • Gene Expression Regulation / drug effects*
  • Gene Expression Regulation, Neoplastic / drug effects
  • Gene Library
  • Genes*
  • Humans
  • Molecular Sequence Data
  • Neoplasm Proteins / biosynthesis
  • Neoplasm Proteins / genetics*
  • Organ Specificity
  • Pregnancy
  • Pregnancy Proteins / biosynthesis
  • Pregnancy Proteins / genetics*
  • Trophoblasts / metabolism*
  • Tumor Cells, Cultured
  • Uterine Neoplasms / genetics*
  • Uterine Neoplasms / metabolism
  • Uterine Neoplasms / pathology

Substances

  • DNA, Complementary
  • Neoplasm Proteins
  • Pregnancy Proteins
  • 8-Bromo Cyclic Adenosine Monophosphate
  • Cyclic AMP

Associated data

  • GENBANK/L13197