Abstract
A partial cDNA (D21S418E) whose nucleotide sequence has no significant homologies with known mammalian DNA sequences was isolated from a human placental library. The cDNA hybridized with a 10-kb transcript present in term placenta. Messages of 10 and 7.5 kb were induced in BeWo and JEG-3 choriocarcinoma cells by treatment with 8-Br-cAMP. The mRNA was not detected in human brain, liver, lung, kidney, pancreas, heart, skeletal muscle, or myometrium. The D21S418E locus was assigned to a 3.5-Mb region of chromosome 21q22.3.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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8-Bromo Cyclic Adenosine Monophosphate / pharmacology
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Animals
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Base Sequence
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Choriocarcinoma / genetics*
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Choriocarcinoma / metabolism
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Choriocarcinoma / pathology
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Chromosome Mapping
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Chromosomes, Human, Pair 21*
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Cricetinae
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Cricetulus
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Cyclic AMP / physiology*
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DNA, Complementary / genetics
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Female
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Gene Expression Regulation / drug effects*
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Gene Expression Regulation, Neoplastic / drug effects
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Gene Library
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Genes*
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Humans
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Molecular Sequence Data
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Neoplasm Proteins / biosynthesis
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Neoplasm Proteins / genetics*
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Organ Specificity
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Pregnancy
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Pregnancy Proteins / biosynthesis
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Pregnancy Proteins / genetics*
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Trophoblasts / metabolism*
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Tumor Cells, Cultured
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Uterine Neoplasms / genetics*
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Uterine Neoplasms / metabolism
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Uterine Neoplasms / pathology
Substances
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DNA, Complementary
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Neoplasm Proteins
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Pregnancy Proteins
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8-Bromo Cyclic Adenosine Monophosphate
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Cyclic AMP