Differentiation of membrane IgE+ rat B cells into IgE-secreting cells

Immunology. 1993 Aug;79(4):580-6.

Abstract

Rat spleen cells were stimulated with pokeweed mitogen (PWM) and the IgM and IgE responses were assessed. An enrichment of the cell suspension with IgE-bearing cells before stimulation resulted in an increase in the number of IgE-secreting cells. A decrease of the number of IgE-secreting cells was found after depletion of IgE- or IgM-bearing cells, but not those bearing IgD molecules on their membranes, before stimulation. Moreover, the stimulation of membrane IgE on B cells with anti-IgE antibodies was shown to increase the number of IgE-secreting cells after PWM-induced differentiation in vitro. In vivo, it was also observed that a single injection of anti-IgE antibodies can induce the differentiation of IgE-secreting cells. These results demonstrate the presence of IgE(+)-IgM (+)-IgD- B cells in the rat that are responsive to PWM-induced differentiation into IgE-secreting cells. They indicate a pre-commitment of these cells at a stage where they still express IgM on their surface. IgE molecules on the cell membranes play a role in their differentiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • B-Lymphocytes / immunology*
  • Cell Differentiation / immunology
  • Cell Membrane / immunology
  • Cells, Cultured
  • Enzyme-Linked Immunosorbent Assay
  • Immunoglobulin D / biosynthesis
  • Immunoglobulin E / biosynthesis*
  • Immunoglobulin Fab Fragments / immunology
  • Immunoglobulin M / biosynthesis
  • Mice
  • Pokeweed Mitogens / immunology
  • Rats
  • Spleen / immunology

Substances

  • Antibodies, Monoclonal
  • Immunoglobulin D
  • Immunoglobulin Fab Fragments
  • Immunoglobulin M
  • Pokeweed Mitogens
  • Immunoglobulin E