Detection of HIV in human vitreous

Int Ophthalmol. 1993 Apr;17(2):101-4. doi: 10.1007/BF00942783.


Assay of human vitreous specimens obtained postmortem for HIV antibodies, or HIV p24 antigen, is reported to be a reliable technique to demonstrate HIV infection in possible cornea donors from whom serum could not be obtained. We tested three vitreous samples obtained during vitrectomy from two HIV-positive patients. One patient exhibited the clinical AIDS syndrome. HIV antigen and antibody tests were negative in all specimens. HIV proviral DNA was detected by PCR only in the vitreous of the patient with AIDS. Therefore, testing only vitreous samples is insufficient to exclude HIV infection in potential cornea donors.

Publication types

  • Case Reports

MeSH terms

  • Adult
  • Base Sequence
  • DNA, Viral / analysis
  • Eye Infections, Viral / microbiology*
  • HIV / genetics
  • HIV / immunology
  • HIV / isolation & purification*
  • HIV Antibodies / analysis
  • HIV Core Protein p24 / analysis
  • HIV Infections / microbiology*
  • Humans
  • Male
  • Molecular Sequence Data
  • Polymerase Chain Reaction / methods
  • Proviruses / genetics
  • Proviruses / immunology
  • Retinal Detachment / surgery
  • Vitrectomy
  • Vitreous Body / immunology
  • Vitreous Body / microbiology*


  • DNA, Viral
  • HIV Antibodies
  • HIV Core Protein p24