HDL-C, LDL-C, total cholesterol and glycerol were determined in venous blood of 10 male trained swimmers at rest and following 100-m anaerobic swimming. When compared to rest levels, the peak HDL-C and glycerol concentrations were significantly enhanced following anaerobic swim test (p < 0.01). However, peak LDL-C and total cholesterol after anaerobic swim were not significantly increased compared with the rest level. The present data demonstrated that the anaerobic swimming induces an increase in HDL-C metabolism, suggesting that the anaerobic exercise per se was one reason for the elevated HDL-C levels.