Crystal Structure of a Yeast TBP/TATA-box Complex

Nature. 1993 Oct 7;365(6446):512-20. doi: 10.1038/365512a0.

Abstract

The 2.5 A crystal structure of a TATA-box complex with yeast TBP shows that the eight base pairs of the TATA box bind to the concave surface of TBP by bending towards the major groove with unprecedented severity. This produces a wide open, underwound, shallow minor groove which forms a primarily hydrophobic interface with the entire under-surface of the TBP saddle. The severe bend and a positive writhe radically alter the trajectory of the flanking B-form DNA.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Base Sequence
  • Computer Graphics
  • Crystallography, X-Ray
  • Cytochrome c Group / genetics
  • Cytochromes c*
  • DNA / chemistry
  • Hydrogen Bonding
  • Molecular Sequence Data
  • Nucleic Acid Conformation
  • Promoter Regions, Genetic
  • Protein Binding
  • Protein Conformation
  • Recombinant Proteins / chemistry
  • Saccharomyces cerevisiae / chemistry
  • Saccharomyces cerevisiae Proteins*
  • Structure-Activity Relationship
  • TATA Box*
  • Transcription Factor TFIIA
  • Transcription Factor TFIID
  • Transcription Factors / chemistry*
  • Transcription Factors / metabolism

Substances

  • CYC1 protein, S cerevisiae
  • Cytochrome c Group
  • Recombinant Proteins
  • Saccharomyces cerevisiae Proteins
  • Transcription Factor TFIIA
  • Transcription Factor TFIID
  • Transcription Factors
  • Cytochromes c
  • DNA