Fascin, an echinoid actin-bundling protein, is a homolog of the Drosophila singed gene product

Proc Natl Acad Sci U S A. 1993 Oct 1;90(19):9115-9. doi: 10.1073/pnas.90.19.9115.

Abstract

A cDNA for fascin, an actin-bundling protein in echinoderms, has been cloned, sequenced, and expressed. The predicted mass of the protein is approximately 55 kDa, similar to that observed for fascin purified from sea urchin eggs. Bacterially expressed fascin reacts with antibodies prepared against sea urchin egg fascin. Fascin has a strong sequence similarity to the singed gene (sn) product in Drosophila and has similarities with a 55-kDa human actin-bundling protein. No extensive similarities were found with other known actin-binding/bundling proteins, indicating that this is a separate gene family.

Publication types

  • Comparative Study
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Carrier Proteins / biosynthesis
  • Carrier Proteins / chemistry
  • Carrier Proteins / genetics*
  • Cloning, Molecular
  • DNA Primers
  • Drosophila melanogaster / genetics*
  • Escherichia coli
  • Female
  • Insect Hormones / chemistry
  • Insect Hormones / genetics*
  • Microfilament Proteins / genetics*
  • Molecular Sequence Data
  • Multigene Family
  • Ovum / metabolism*
  • Polymerase Chain Reaction
  • Restriction Mapping
  • Sea Urchins / genetics*
  • Sequence Homology, Amino Acid

Substances

  • Carrier Proteins
  • DNA Primers
  • Insect Hormones
  • Microfilament Proteins
  • fascin

Associated data

  • GENBANK/L12047