Expression of a cDNA encoding the glycolipid-anchored form of rat acetylcholinesterase

FEBS Lett. 1993 Jan 4;315(2):163-6. doi: 10.1016/0014-5793(93)81155-s.


We amplified by PCR and characterized a fragment of cDNA from rat spleen, encoding the distinctive C-terminal region of the acetylcholinesterase (AChE) H subunit. A recombinant vector encoding this subunit was constructed and expressed in COS cells: the H subunits produced glycophosphatidylinositol (GPI)-anchored dimers, showing that the spleen cDNA fragment contained a functional GPI cleavage/attachment site. Using PCR, we did not detect mRNAs encoding AChE H in rat muscle or hypothalamus. In the liver of 16-day rat embryos, we found both H and T transcripts, in agreement with the presence of both GPI-anchored dimers and amphiphilic monomers of type II. In addition, we detected 'read-through' (R) transcripts, in which regular introns are spliced, but the intervening sequence between the common exon 4 and the alternative exon 5 (H) is maintained.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholinesterase / genetics*
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Line
  • Chlorocebus aethiops
  • Cloning, Molecular
  • DNA / genetics
  • Gene Expression
  • Glycosylphosphatidylinositols / metabolism*
  • Molecular Sequence Data
  • Oligodeoxyribonucleotides / chemistry
  • Polymerase Chain Reaction
  • RNA Splicing
  • RNA, Messenger / genetics
  • Rats


  • Glycosylphosphatidylinositols
  • Oligodeoxyribonucleotides
  • RNA, Messenger
  • DNA
  • Acetylcholinesterase

Associated data

  • GENBANK/X68456
  • GENBANK/X68457
  • GENBANK/X68458
  • GENBANK/X68459
  • GENBANK/X68460
  • GENBANK/X68461
  • GENBANK/X68462
  • GENBANK/X68463
  • GENBANK/X70140
  • GENBANK/X70141