Regulation of Cu,Zn- and Mn-superoxide dismutase transcription in Saccharomyces cerevisiae

FEBS Lett. 1993 Jan 4;315(2):197-200. doi: 10.1016/0014-5793(93)81162-s.

Abstract

The regulation of Cu,Zn- and Mn-superoxide dismutases (SOD) was investigated by Northern blotting and gene fusions of SOD1 and SOD2 promoters with the beta-galactosidase reporter gene. Cu,ZnSOD expression was increased 3-fold under glucose derepressing conditions, and decreased 4- to 6-fold by oxygen or heme deficiency. MnSOD expression was increased 5-fold by glucose derepression, and decreased 8- to 10-fold by anaerobiosis and 4- to 5-fold by heme deficiency. Induction by paraquat was modest, about 50% for SOD1 and 100% for SOD2; it was apparently independent of the respiratory chain function.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Carbon / metabolism
  • Gene Expression Regulation, Fungal* / drug effects
  • Heme / metabolism
  • Hot Temperature
  • Oxygen / pharmacology
  • Paraquat / pharmacology
  • RNA, Messenger / genetics
  • Saccharomyces cerevisiae / enzymology*
  • Saccharomyces cerevisiae / genetics
  • Saccharomyces cerevisiae / growth & development
  • Superoxide Dismutase / genetics*
  • Transcription, Genetic

Substances

  • RNA, Messenger
  • Heme
  • Carbon
  • Superoxide Dismutase
  • Paraquat
  • Oxygen