Gene II product of an aphid-nontransmissible isolate of cauliflower mosaic virus expressed in a baculovirus system possesses aphid transmission factor activity

Virology. 1993 Feb;192(2):651-4. doi: 10.1006/viro.1993.1081.


A new baculovirus expression system was used to express the gene II which encodes the 18-kDa aphid transmission factor (ATF) protein of two isolates of cauliflower mosaic virus (CaMV), the aphid-transmissible (AT+) Cabb B-JI and the aphid-nontransmissible (AT-) CM1841 isolates. ATF activity was shown to be associated with the baculovirus expression product of CaMV gene II from both the AT+ and the AT- isolates. No differences could be detected in the stability of the P18s from AT+ and AT- isolates expressed in the baculovirus system. These results indicate that the AT- phenotype of CM1841 isolate might not be due to a defective P18 but to an insufficient concentration of it in infected plant tissue.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Aphids
  • Baculoviridae / genetics*
  • Brassica
  • Cell Line
  • Genes, Viral*
  • Insecta
  • Mosaic Viruses / genetics*
  • Plant Proteins / biosynthesis
  • Plant Proteins / genetics*
  • Restriction Mapping
  • Transfection
  • Viral Proteins / biosynthesis
  • Viral Proteins / genetics*


  • Plant Proteins
  • Viral Proteins