Saccharomyces Telomeres Acquire Single-Strand TG1-3 Tails Late in S Phase

Cell. 1993 Jan 15;72(1):51-60. doi: 10.1016/0092-8674(93)90049-v.

Abstract

Saccharomyces telomeres consist of approximately 300 bp of C1-3A/TG1-3 DNA. Nondenaturing Southern hybridization, capable of detecting approximately 60 to approximately 300 bases of TG1-3 DNA, revealed that yeast telomeres acquired and lost TG1-3 tails, the predicted intermediate in telomere replication, in a cell cycle-dependent manner. TG1-3 tails were also detected on the ends of a linear plasmid isolated from late S phase cells. In addition, a nonlinear form of this plasmid was detected: this structure migrated in two-dimensional agarose gels like a nicked circle of the same size as the linear plasmid, but had considerably more single-stranded character than a conventional nicked circle. The evidence indicates that these circles were formed by telomere-telomere interactions involving the TG1-3 tails. These data provide evidence for a cell cycle-dependent change in telomere structure and demonstrate that TG1-3 tails, generated during replication of a linear plasmid in vivo, are capable of mediating telomere-telomere interactions.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Base Composition
  • Blotting, Southern
  • Chromosomes, Fungal
  • DNA, Fungal / genetics*
  • DNA, Single-Stranded / genetics*
  • Electrophoresis, Agar Gel
  • Electrophoresis, Gel, Two-Dimensional
  • Plasmids
  • Repetitive Sequences, Nucleic Acid
  • S Phase / genetics*
  • Saccharomyces / cytology
  • Saccharomyces / genetics*
  • Telomere*

Substances

  • DNA, Fungal
  • DNA, Single-Stranded