Thiamine-repressible Expression Vectors pREP and pRIP for Fission Yeast

Gene. 1993 Jan 15;123(1):127-30. doi: 10.1016/0378-1119(93)90551-d.

Abstract

The promoter and polyadenylation signal of the thiamine-repressible gene nmt1 of Schizosaccharomyces pombe have been used to construct the pREP extrachromosomally replicating plasmids and the pRIP integrative expression plasmids. These plasmids permit thiamine-mediated control of transcription to be applied to cloned genes.

MeSH terms

  • Base Sequence
  • Cloning, Molecular
  • DNA, Fungal
  • Gene Expression Regulation, Fungal
  • Genetic Vectors*
  • Molecular Sequence Data
  • Plasmids*
  • Restriction Mapping
  • Schizosaccharomyces / genetics*
  • Schizosaccharomyces / metabolism
  • Thiamine / metabolism*

Substances

  • DNA, Fungal
  • Thiamine