The whole structure of the gene for phenylalanine ammonia-lyase of rice, designated as GP-28, was determined. The gene included one intron located between nucleotides 557 and 2048, corresponding to the second and third letters, respectively, of the arginine codon at position 136 which is conserved in the genes of other plant species. The open reading frame was capable of encoding a 716 amino acid sequence, which showed 73.3% similarity with that of parsley. In the carboxyl-terminal region, an arginine-rich sequence common to the rice enzyme was found, which showed low similarity with those of dicot plants.