Inositol trisphosphate restores impaired human gallbladder motility associated with cholesterol stones

Gastroenterology. 1993 Feb;104(2):563-8. doi: 10.1016/0016-5085(93)90427-e.


Background: Gallbladder motility is impaired in specimens with cholesterol stones but normal with pigment stones.

Methods: Muscle cells obtained from 19 human gallbladders with cholesterol stones and 11 with pigment stones were enzymatically digested and contracted with cholecystokinin octapeptide (CCK-8), acetylcholine, and KCl.

Results: Muscle cells from pigment stones had a greater contraction than cells from cholesterol stones. CCK-8-induced contraction was unaffected by calcium-free media but was blocked by strontium. Potassium-evoked contraction was blocked by a calcium-free media and unaffected by strontium. Inositol triphosphate (IP-3)-induced contraction was similar to the contraction caused by CCK-8 in permeable cells from pigment stones but was greater than the response to CCK-8 in cells from cholesterol stones.

Conclusions: Muscle cells from gallbladders with cholesterol stones contract less than cells from gallbladders with pigment stones; CCK-8-induced contraction only uses stored calcium; and IP-3 causes contractions of equal magnitude in cells from gallbladders with cholesterol and pigment stones. These abnormalities could result from an impaired receptor activation of the mechanism for IP-3 generation and release of stored calcium.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acetylcholine / pharmacology
  • Adult
  • Aged
  • Calcium / metabolism
  • Cholelithiasis / physiopathology*
  • Cholesterol / metabolism*
  • Female
  • Gallbladder / drug effects
  • Gallbladder / physiopathology*
  • Humans
  • In Vitro Techniques
  • Inositol 1,4,5-Trisphosphate / pharmacology*
  • Male
  • Middle Aged
  • Muscle Contraction / drug effects*
  • Potassium / pharmacology
  • Sincalide / pharmacology


  • Inositol 1,4,5-Trisphosphate
  • Cholesterol
  • Sincalide
  • Acetylcholine
  • Potassium
  • Calcium