Characterisation of dog allergens by means of immunoblotting

Int Arch Allergy Immunol. 1993;100(1):60-7. doi: 10.1159/000236389.

Abstract

Sera from 75 patients with clinical type I allergy against dogs were investigated by means of immunoblotting using extracts prepared from dog hair/dander (CAN XI D) and saliva. In addition, selected sera were tested on extracts made of hair, skin, salivary glands (parotis and submandibularis), serum and liver. A 69-kD IgE-binding protein was identified in all extracts tested with an incidence of approximately 40% and shown to be dog albumin by means of inhibition experiments. In 96% of patients' sera IgE antibodies reactive with a 19-kD and/or a 23-kD protein of the hair/dander extract (CAN XI D) were observed. IgE binding to a 23-kD band was also detected in the hair and saliva extracts, but not in skin, salivary gland, serum and liver extracts. A 19-kD IgE-binding protein was strongly expressed in skin and to a lesser degree in saliva, but not in hair, serum and liver. Preincubation of patients sera with the hair extract and subsequent probing with the hair/dander extract (CAN XI D) inhibited IgE binding to the 23 kD protein whereas preincubation with the skin extract abolished IgE binding to the 19-kD protein. Using the hair/dander extract as inhibitor, IgE binding to the 19- and 23-kD proteins of saliva was abrogated. Thus it is concluded that the 23-kD protein is preferentially expressed in hair and saliva whereas the 19-kD protein is found in saliva and skin. Furthermore these two proteins are likely to represent immunologically independent major allergens.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adolescent
  • Adult
  • Allergens / chemistry*
  • Animals
  • Antigen-Antibody Reactions
  • Child
  • Dogs / immunology*
  • Female
  • Hair / immunology
  • Humans
  • Immunoblotting
  • Immunoglobulin E / immunology
  • Liver / immunology
  • Male
  • Middle Aged
  • Radioallergosorbent Test
  • Saliva / immunology
  • Skin / immunology
  • Tissue Extracts / immunology

Substances

  • Allergens
  • Tissue Extracts
  • Immunoglobulin E