Phenotypic and molecular analysis of Ph1-chromosome-positive acute lymphoblastic leukemia cell lines

Int J Cancer. 1993 Feb 1;53(3):457-62. doi: 10.1002/ijc.2910530318.


We have established 2 Philadelphia chromosome (Ph1)-positive acute lymphoblastic leukemia (ALL) cell lines, designated PALL-1 and PALL-2, from distinct adult Ph1-positive ALL patients. PALL-1 was established in nude mice, and PALL-2 was established in culture. Both retained the Ph1 chromosome and expressed the ALL type bcr/abl chimeric mRNA containing the junction of the first exon of BCR gene (e1) and second exon of c-abl gene (a2). PALL-1 and PALL-2 expressed CD34 surface antigen which is characteristic of early hematopoietic progenitor cells. PALL-2 expressed antigens for both pre-B and early myeloid cells and had rearrangements of both the heavy chain of immunoglobulin gene and the beta chain of T-cell-receptor gene. Both PALL-1 and PALL-2 expressed detectable levels of p53 gene RNA. Polymerase-chain-reaction-single-strand conformation polymorphism (PCR-SSCP) analysis of the p53 gene showed a normal pattern of mobility in both cell lines. Taken together, the 2 cell lines had features of Ph1-positive ALL: (i) hematopoietic progenitor cells with pre-B-cell phenotype and, (ii) activation of e1-a2 type bcr/abl oncogene without alterations of p53 gene. These unique lines should provide a valuable tool for studying the pathogenesis of Ph1-positive ALL.

Publication types

  • Case Reports
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adult
  • Animals
  • Base Sequence
  • Fusion Proteins, bcr-abl
  • Gene Rearrangement, B-Lymphocyte, Heavy Chain
  • Genes, abl
  • Genes, p53
  • Humans
  • Male
  • Mice
  • Mice, Nude
  • Neoplasm Transplantation
  • Oligodeoxyribonucleotides / chemistry
  • Philadelphia Chromosome*
  • Precursor Cell Lymphoblastic Leukemia-Lymphoma / pathology*
  • Protein-Tyrosine Kinases*
  • Proto-Oncogene Proteins / genetics
  • Proto-Oncogene Proteins c-bcr
  • Receptors, Antigen, T-Cell, alpha-beta / genetics
  • Tumor Cells, Cultured / pathology*


  • Oligodeoxyribonucleotides
  • Proto-Oncogene Proteins
  • Receptors, Antigen, T-Cell, alpha-beta
  • Protein-Tyrosine Kinases
  • Fusion Proteins, bcr-abl
  • BCR protein, human
  • Bcr protein, mouse
  • Proto-Oncogene Proteins c-bcr