Abstract
Melanoma formation in the teleost Xiphophorus is caused by a dominant genetic locus, Tu. This locus includes the Xmrk oncogene, which encodes a receptor tyrosine kinase. Tumor induction is suppressed in wild-type fish by a tumor suppressor locus, R. Molecular genetic analyses revealed that the Tu locus emerged by nonhomologous recombination of the Xmrk proto-oncogene with a previously uncharacterized sequence, D. This event generated an additional copy of Xmrk with a new promoter. Suppression of the new Xmrk promoter by R in parental fish and its deregulation in hybrids explain the genetics of melanoma formation in Xiphophorus.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Sequence
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Animals
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Base Sequence
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Cyprinodontiformes / genetics*
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Embryo, Nonmammalian
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Fish Diseases / genetics*
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Fish Proteins*
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Gene Library
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Genes, Tumor Suppressor*
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Melanoma / genetics
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Melanoma / veterinary*
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Molecular Sequence Data
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Oligodeoxyribonucleotides
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Oncogenes*
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Polymerase Chain Reaction / methods
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Promoter Regions, Genetic*
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Protein-Tyrosine Kinases / genetics*
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Proto-Oncogenes
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Receptor Protein-Tyrosine Kinases*
Substances
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Fish Proteins
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Oligodeoxyribonucleotides
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Protein-Tyrosine Kinases
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Receptor Protein-Tyrosine Kinases
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Xmrk protein, Xiphophorus
Associated data
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GENBANK/L08396
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GENBANK/L08397
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GENBANK/L08398