Selective spore survival during replica-plating of fission yeast

Arch Microbiol. 1977 Feb 4;112(1):109-10. doi: 10.1007/BF00446662.

Abstract

Vegetative cells of Schizosaccharomyces pombe, upon exposure to acetone vapours, are inactivated at a faster rate than ascospores of this yeast. This observation has been used to develop a simple and fast method by which colonies can be replica-plated and only spores survive in the replicas. The colony patterns are exposed to acetone while still on the velvet used for replica-plating.

MeSH terms

  • Acetone
  • Ascomycota / isolation & purification*
  • Methods
  • Schizosaccharomyces / isolation & purification*
  • Spores, Fungal / isolation & purification

Substances

  • Acetone