Interferon-gamma promotes proliferation of rat skeletal muscle cells in vitro and alters their AChR distribution

J Neurol Sci. 1993 Jan;114(1):62-7. doi: 10.1016/0022-510x(93)90050-9.


Recombinant interferon-gamma (IFN-gamma) caused a dose-dependent increase in the proliferation of myoblasts in cultures of rat skeletal muscles as determined by bromodeoxyuridine incorporation. As a result of this proliferation the number of myotubes increased in the cultures, while the fusion index was unchanged. In myotubes exposed to IFN-gamma there was a change in the distribution of nicotinic acetylcholine receptors (nAChR) as detected by binding of FITC-conjugated alpha-bungarotoxin with a significant decrease in the number of linear aggregates and an increase in diffusely distributed receptors.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Acetylcholinesterase / metabolism*
  • Animals
  • Cell Division / drug effects
  • Cell Fusion / drug effects
  • Cell Nucleus / drug effects
  • Female
  • In Vitro Techniques
  • Interferon-gamma / pharmacology*
  • Microtubules / drug effects
  • Muscles / cytology*
  • Muscles / drug effects
  • Muscles / enzymology
  • Pregnancy
  • Rats
  • Rats, Sprague-Dawley
  • Receptors, Nicotinic / drug effects


  • Receptors, Nicotinic
  • Interferon-gamma
  • Acetylcholinesterase