Standard system for infecting ticks (Acari: Ixodidae) with the Lyme disease spirochete, Borrelia burgdorferi

J Med Entomol. 1993 Jan;30(1):199-203. doi: 10.1093/jmedent/30.1.199.


A standard system for infecting ticks with the Lyme disease spirochete Borrelia burgdorferi Johnson, Schmid, Hyde, Steigerwalt & Brenner is defined. Rodents infected via tick feeding or inoculation of tick homogenates were more infectious to ticks than rodents infected with culture-derived spirochetes. White laboratory mice were more infectious than hamsters. Three strains of B. burgdorferi (JD1, B31, and WI210) produced batches of infected ticks with > 80% infection when mice were infected with tick-derived material. Ixodes dammini Spielman, Clifford, Piesman & Corwin were 3.6x more efficient than I. pacificus Cooley & Kohls in acquiring and maintaining infection with two California strains of B. burgdorferi, originally isolated from I. pacificus.

MeSH terms

  • Animals
  • Arachnid Vectors / microbiology*
  • Borrelia burgdorferi Group / growth & development*
  • Borrelia burgdorferi*
  • Cricetinae
  • Female
  • Male
  • Mesocricetus
  • Mice
  • Ticks / microbiology*