Expression of IP-10, a lipopolysaccharide- and interferon-gamma-inducible protein, in murine mesangial cells in culture

Am J Pathol. 1993 Feb;142(2):433-9.

Abstract

IP-10 is an early gene induced in multiple cell types by a variety of proinflammatory agents, notably interferons (IFNs) and lipopolysaccharide (LPS). To determine whether this protein might play a role in amplifying immune-mediated glomerular injury, we cultured mouse mesangial cells with several stimuli for various times. Increasing amounts of IFN-gamma (to 100 units/ml) elicited increasing levels of IP-10 messenger RNA (mRNA), sustained to 24 hours, but had no effect on tumor necrosis factor-alpha (TNF-alpha) mRNA. LPS induced transient IP-10 mRNA expression that peaked at 8 hours; TNF-alpha mRNA was also increased. TNF-alpha at doses up to 10 ng/ml and soluble immune complexes up to 150 micrograms/ml antibody evoked 3- to 5-fold increases in IP-10 mRNA expression, much less than the 30- to 70-fold increases seen with IFN-gamma and LPS. We conclude that IFN-gamma, LPS, and other agonists can amplify glomerular immune injury, perhaps via elevated expression of IP-10.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Animals
  • Cells, Cultured
  • Chemokine CXCL10
  • Chemokines, CXC*
  • Cycloheximide / pharmacology
  • Cytokines / genetics
  • Cytokines / metabolism*
  • Dose-Response Relationship, Drug
  • Glomerular Mesangium / cytology
  • Glomerular Mesangium / metabolism*
  • Interferon-gamma / pharmacology*
  • Lipopolysaccharides / pharmacology*
  • Mice
  • RNA, Messenger / metabolism
  • Time Factors

Substances

  • Chemokine CXCL10
  • Chemokines, CXC
  • Cytokines
  • Lipopolysaccharides
  • RNA, Messenger
  • Interferon-gamma
  • Cycloheximide