Design and construction of a linear shear stress flow chamber

Ann Biomed Eng. 1993;21(1):77-83. doi: 10.1007/BF02368167.

Abstract

A new parallel plate flow chamber that has a linear variation of shear stress, starting from a predetermined maximum value at the entrance and falling to zero at the exit, has been designed and tested. This is in contrast to the usual rectangular channel plan which produces a constant shear stress over the entire length. The new design is based on the theory of Hele-Shaw flow between parallel plates. To verify the efficacy of the flow channel, the effect of fluid shear stress on platelet adhesion to a fibrinogen-coated glass surface was tested. The percentage of attached platelets after 5 min of shear stress is shown to be a function of shear stress. With this new flow chamber, cell-cell interactions can be studied efficiently over a wide range of shear stress using a single run at constant discharge.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Endothelium, Vascular / physiology*
  • Equipment Design / standards*
  • Evaluation Studies as Topic
  • Fibrinogen / physiology*
  • Glass
  • Humans
  • Microscopy
  • Platelet Adhesiveness / physiology*
  • Rheology / instrumentation*
  • Rheology / standards
  • Videotape Recording

Substances

  • Fibrinogen