A rapid method for measuring serum amyloid A protein by latex agglutination nephelometric immunoassay

Ann Clin Biochem. 1993 Jan;30 ( Pt 1):72-6. doi: 10.1177/000456329303000112.

Abstract

Serum amyloid A protein (SAA) is a sensitive acute phase reactant. We developed a method for the rapid measurement of human SAA in serum by kinetic nephelometry of anti-SAA antibody-coated latex agglutination. Measurement takes less than 6 min using an automated analyser. Standardization of the assay employs SAA-enriched high density lipoprotein as the primary standard. The values determined by our new method and by conventional enzyme-immunoassay showed good agreement (r = 0.988). The normal range was 0.17-10.0 mg/L [mean(SD)]. This rapid method should prove useful in clinical laboratories.

MeSH terms

  • Antibodies, Monoclonal
  • Calibration
  • Electrophoresis, Polyacrylamide Gel
  • Humans
  • Immunoenzyme Techniques
  • Latex Fixation Tests* / standards
  • Lipoproteins, HDL / chemistry
  • Nephelometry and Turbidimetry* / standards
  • Reference Standards
  • Reference Values
  • Serum Amyloid A Protein / analysis*

Substances

  • Antibodies, Monoclonal
  • Lipoproteins, HDL
  • Serum Amyloid A Protein