Effect of bile acids on intracellular calcium in isolated rat hepatocyte couplets

Biochem Pharmacol. 1993 Jan 26;45(2):289-93. doi: 10.1016/0006-2952(93)90063-3.

Abstract

The effects of bile acids on cytosolic free calcium ([Ca2+]i) were studied in single isolated rat hepatocyte couplets using a scanning laser cytometer and the fluorescent dye, indo-1. Cholestatic bile acids, chenodeoxycholate (CDC) and taurolithocholate (TLC) increased [Ca2+]i in a dose-dependent manner. Choleretic bile acids, tauroursodeoxycholate (TUDC) and taurocholate (TC), did not induce any change in [Ca2+]i except TC at very high doses. Treatment with TUDC added concomitantly with CDC or TLC significantly decreased the rise in [Ca2+]i induced by bile acids. These results, obtained with a polarized hepatocyte model that secretes bile, confirmed that cholestatic bile acids increase [Ca2+]i and showed that TUDC inhibits this phenomenon. These data are in agreement with a key role of intracellular calcium in cholestasis.

Publication types

  • Comparative Study

MeSH terms

  • Animals
  • Bile Acids and Salts / pharmacology*
  • Calcium / metabolism*
  • Chenodeoxycholic Acid / pharmacology
  • In Vitro Techniques
  • Liver / cytology
  • Liver / drug effects*
  • Liver / metabolism
  • Male
  • Models, Biological
  • Rats
  • Rats, Sprague-Dawley
  • Taurochenodeoxycholic Acid / pharmacology
  • Taurolithocholic Acid / pharmacology
  • Vasopressins / pharmacology

Substances

  • Bile Acids and Salts
  • Chenodeoxycholic Acid
  • Vasopressins
  • Taurochenodeoxycholic Acid
  • Taurolithocholic Acid
  • ursodoxicoltaurine
  • Calcium