Co-localization of two calcium binding proteins in GABA cells of rat piriform cortex

Brain Res. 1993 Jan 15;600(2):339-44. doi: 10.1016/0006-8993(93)91394-8.


In the rat piriform cortex, double-fluorescence immunocytochemistry was used to study the coexistence of two calcium binding proteins, parvalbumin and calbindinD28k, and GABA. Layer I cells were immunoreactive only for GABA. In layer II a small number of cells were immunoreactive for all three substances. A few other cells contained only parvalbumin and GABA immunoreactivity. In layer III a subpopulation of calbindinD28k immunoreactive cells (about 60%) were also immunoreactive for both parvalbumin and GABA. The remainder showed only calbindinD28k and GABA immunoreactivity. Many immunoreactive terminals, which surrounded non-immunoreactive cell somata to form basket endings in layers II and III, were immunoreactive for all three substances. Their major source may be the non-pyramidal neurons in layer III, which contained the three chemical substances.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Monoclonal / immunology
  • Calbindins
  • Calcium-Binding Proteins / metabolism*
  • Cerebral Cortex / anatomy & histology
  • Cerebral Cortex / cytology
  • Cerebral Cortex / metabolism*
  • Cross Reactions
  • Immunohistochemistry
  • Male
  • Nerve Fibers / immunology
  • Nerve Fibers / metabolism
  • Neurons / metabolism
  • Neurons / physiology*
  • Parvalbumins / metabolism
  • Rats
  • Rats, Wistar
  • S100 Calcium Binding Protein G / metabolism
  • gamma-Aminobutyric Acid / physiology*


  • Antibodies, Monoclonal
  • Calbindins
  • Calcium-Binding Proteins
  • Parvalbumins
  • S100 Calcium Binding Protein G
  • gamma-Aminobutyric Acid