Stable expression and regulation of a rat brain K+ channel

J Neurochem. 1993 Mar;60(3):1175-8. doi: 10.1111/j.1471-4159.1993.tb03273.x.


The Shaw-type K+ channel Kv3.1 was stably transfected in human embryonic kidney cells. Voltage dependence of activation, K+ permeability, sensitivity to external tetraethylammonium, and unitary conductance were similar to Kv3.1 channels expressed transiently in Xenopus oocytes. Kv3.1 channels appear to be regulated because the protein kinase C activator phorbol 12,13-dibutyrate decreased Kv3.1 currents. Based on these results, we find that the stable expression of voltage-gated K+ channels in human embryonic kidney cells appears to be well suited for analysis of both biophysical and biochemical regulatory processes.

MeSH terms

  • Animals
  • Brain / metabolism*
  • Cell Line, Transformed
  • Electrophysiology
  • Enzyme Activation
  • Humans
  • Kidney / cytology
  • Kidney / metabolism
  • Kidney / physiology
  • Phorbol 12,13-Dibutyrate / pharmacology
  • Potassium Channels / metabolism*
  • Potassium Channels / physiology
  • Protein Kinase C / metabolism
  • Rats
  • Transfection


  • Potassium Channels
  • Phorbol 12,13-Dibutyrate
  • Protein Kinase C