Physical linkage of the SLG and SRK genes at the self-incompatibility locus of Brassica oleracea

Mol Gen Genet. 1993 Jan;236(2-3):369-73. doi: 10.1007/BF00277135.


In Brassica oleracea, the pollen-stigma interaction of self-incompatibility is controlled by a single genetically defined locus designated S. Molecular studies have identified two genes that are tightly linked to the classically defined S locus: The S-Locus Glycoprotein (SLG) gene and the S-Receptor Kinase (SRK) gene. In previous RFLP linkage analyses with probes specific for SLG and SRK, we were unable to identify any recombination events between SLG, SRK, and self-incompatibility phenotype. In this paper, we use pulsed-field gel electrophoresis (PFGE) in conjunction with DNA blot analysis to characterize the S-locus region from two highly divergent self-incompatibility genotypes, S2 and S6. We establish the physical linkage of SLG and SRK in each genotype, and demonstrate that the two genes are separated by a maximum distance of 220 kb in the S6 genotype and 350 kb in the S2 genotype. Furthermore, a comparison of the data from the two genotypes reveals that a high level of polymorphism exists across the entire S-locus region.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Brassica / genetics*
  • Crosses, Genetic*
  • DNA Probes
  • Deoxyribonucleases, Type II Site-Specific / metabolism
  • Electrophoresis, Gel, Pulsed-Field
  • Genes, Plant / genetics*
  • Genetic Linkage*
  • Glycoproteins / genetics
  • Haplotypes
  • Homozygote
  • Nucleic Acid Hybridization
  • Phenotype
  • Plant Proteins / genetics
  • Pollen / physiology
  • Protein Kinases / genetics


  • DNA Probes
  • Glycoproteins
  • Plant Proteins
  • self-incompatibility locus specific glycoprotein, Brassica
  • Protein Kinases
  • S-receptor kinase
  • Deoxyribonucleases, Type II Site-Specific