Conversion of 2-chloromaleylacetate in Alcaligenes eutrophus JMP134

Arch Microbiol. 1993;159(2):182-8. doi: 10.1007/BF00250280.


2,4-Dichlorophenoxyacetate (2,4-D) in Alcaligenes eutrophus JMP134 (pJP4) is degraded via 2-chloromaleylacetate as an intermediate. The latter compound was found to be reduced by NADH in a maleylacetate reductase catalyzed reaction. Maleylacetate and chloride were formed as products of 2-chloromaleylacetate reduction, the former being funneled into the 3-oxoadipate pathway by a second reductive step. There was no indication for an involvement of a pJP4-encoded enzyme in either the reduction or the dechlorination reaction.

MeSH terms

  • 2,4-Dichlorophenoxyacetic Acid / metabolism*
  • Alcaligenes / enzymology
  • Alcaligenes / metabolism*
  • Biodegradation, Environmental
  • Maleates / metabolism*
  • Oxidoreductases / metabolism*
  • Oxidoreductases Acting on CH-CH Group Donors*


  • Maleates
  • maleoylacetic acid
  • 2-chloromaleylacetate
  • 2,4-Dichlorophenoxyacetic Acid
  • Oxidoreductases
  • Oxidoreductases Acting on CH-CH Group Donors
  • maleylacetate reductase